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Co-Culture Device
Migration Device Large
10 X Ananda Devices
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Neuro Device
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Each package includes :
Migration device Small
Custom Device
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Migration Device Medium
Press Kit
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Axonal Transport of mitochondria (green) in DRG axons
The Neuro Device is a biocompatible silicone insert that enables researchers to easily access neuronal cultures and is ideal for long-term microscopy analysis. The device has a special sticky bottom that prevents leaking on smooth, dry surfaces, such as microscopy slides and dishes. The Neuro Device consists of two chambers connected by microchannels. Cells are seeded in the chambers and the microchannels help to direct axonal extension. Furthermore, different populations can be cultured in the different compartments. - Enables growth of 100 axons with more than 1 mm in length. - Completely transparent and compatible with all fluorescent dyes. - Can be removed any time for direct manipulation of neurons. - Has been used to grow hippocampal, cortical, DRG and sensory neurons from mouse, rat and humans. - The device is 17 mm in diameter - Fit in any 35 mm plastic or glass bottom dishes and in any coverslip with a diameter larger than 18mm - Each pack contains 10 devices and one illustrated user manual
Enables Rewiring of Neuronal Networks
Ambiguous analysis of axonal transport
Precise and Clear Imaging
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Fast and precise analysis of axonal transport
Easy Comparison of Results
Magdesian et al., J. Neuroscience 2016
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3D imaging of axons using Atomic Force Microscopy
Magdesian et al., Biophys. J. 2012
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Enable analysis of Rare Samples
Invasiveness & Metastasis
Needs less than 1,000 cells of a glioblastoma biopsy and 40 microliters of reagents to investigate cancer cell migration.
The Migration Device Small is a biocompatible silicone insert that enables researchers to easily access cellular motility through capillaries smaller than the cell size. This device is ideal for reproducible and comparative analysis of cancer cell deformation, invasion and migration through small compartments. The device has a special sticky bottom that prevents leaking on smooth, dry surfaces, such as microscopy slides and dishes. The Migration Device Small consists of two chambers connected by microchannels. Cells are seeded in the chambers and navigate through the narrow microchannels. Furthermore, different populations can be cultured in the different compartments. - Enables analysis of cell migration through 100 microchannels with more than 1 mm in length. - Completely transparent and compatible with all fluorescent dyes. - Has been used to analyze cancer cell migration. - The device is 17 mm in diameter - Fit in any 35 mm plastic or glass bottom dishes and in any coverslip with a diameter larger than 18mm - Each pack contains 10 devices and one illustrated user manual
The Migration Device Large is a biocompatible silicone insert that enables researchers to easily access cellular motility through large capillaries. This device is ideal for reproducible and comparative analysis of motile cells. The device has a special sticky bottom that prevents leaking on smooth, dry surfaces, such as microscopy slides and dishes. The Migration Device Large consists of two chambers connected by microchannels. Cells are seeded in one chamber and navigate through the microchannels towards a stimuli on the second chamber. Furthermore, different populations can be cultured in the different compartments. - Enables analysis of cell migration through 100 microchannels with more than 1 mm in length. - Completely transparent and compatible with all fluorescent dyes. - Has been used to analyze parasite cell migration. - The device is 17 mm in diameter - Fit in any 35 mm plastic or glass bottom dishes and in any coverslip with a diameter larger than 18mm - Each pack contains 10 devices and one illustrated user manual
MICRODEVICES TO STUDY PARASITE MOTILITY AND MIGRATION
Protozoa parasites (green) migrate through thin channels attracted by specific proteins present in another compartment. The number of parasites in the channels increase with time. Video: R. Tonelli, R. Mortara & F. Real.
Standardized quantification of Immunological response
Drug C
Neurons
Microglia
Culture cells in vitro with similar organization as in vivo. Organize cell cultures with micrometer precision, increasing standardization, precision and reproducibility of cellular assays. Image and analyze cells up to 50% faster due to standard cellular organization reproduced in all dishes. Miniaturize cellular assays, thereby reducing costs with reagents & samples by up to 90%. Incorporate very expensive reagents and analyze rare samples in a larger number of tests due to low reagent’s consumption. Keep neuronal organization for longer than 30 days inside the devices.
Drug D
Not stimulated
Easy quantification of microglia migration towards neurons
Easy quantification and standardization of cell migration, facilitating the comparison of different molecules and its effects on CNS immunological response.
Neurons
Advantages
Drug A
The Migration Device Medium is a biocompatible silicone insert that enables researchers to easily access cellular motility through capillaries with similar size as the cells. This device is ideal for reproducible and comparative analysis of migration of motile cells. The device has a special sticky bottom that prevents leaking on smooth, dry surfaces, such as microscopy slides and dishes. The Migration Device Medium consists of two chambers connected by microchannels. Cells are seeded in one chamber and navigate through the microchannels towards a stimuli on the second chamber. Furthermore, different populations can be cultured in the different compartments. - Enables analysis of cell migration through 80 microchannels with more than 1 mm in length. - Completely transparent and compatible with all fluorescent dyes. - Has been used to analyze glial cell migration. - The device is 17 mm in diameter - Fit in any 35 mm plastic or glass bottom dishes and in any coverslip with a diameter larger than 18mm - Each pack contains 10 devices and one illustrated user manual
24h after stimuli
Drug B
Magdesian et al., J. Neuroscience 2016
The Co-Culture Device Large is a biocompatible silicone insert that enables researchers to easily grow up to 4 individual cell populations in the same dish. This device is ideal for reproducible and comparative analysis of the same cell type with up to 4 different treatments. In addition, the device can be removed at any time to investigate the interactions between the different populations. The device has a special sticky bottom that prevents leaking on smooth, dry surfaces, such as microscopy slides and dishes. The device is completely transparent and can be used during fluorescent imaging. - Enables the study of the interaction of different cell populations. - Completely transparent and compatible with all fluorescent dyes. - Has been used to analyze neuronal networks. - The Co-culture Device is 20 x 15 mm in size with 4 independent 3 x 5 mm compartments - Fit in any 35 mm plastic dish, 35 mm glass bottom dish with 25 mm glass bottom and on any coverslip with a diameter larger than 25 mm. -  Each pack contains 10 devices and one illustrated user manual
Easy analysis of cellular interactions after several days in isolation in response to different stimuli. a) Isolated neuronal populations inside the device. b) Interaction between isolated neuronal population after removal of device with and c) without stimuli.
a
Devices to Rewire Neuronal Networks
CO-CULTURE DEVICE
COO
Product Manager
ANANDA (Advanced Nano Design Applications) is the result of an interdisciplinary collaboration between cell biologists, physicists and engineers from the Montreal Neurological Institute and the McGill Nanotools Microfab. Our goal is to increase research capacity and competitiveness by developing and applying nano- and microtechnology platforms to improve biomedical research and diagnostics. Drug development is slow, expensive and inefficient. Over 60% of biological research cannot be reproduced and scientists still use an outdated cell culture model to test new drugs. Major scientific breakthroughs rely on robust cell cultures, reproducible experiments and reliable results. Ananda Devices bring drug testing to the 21st century. We offer unique silicone microfluidic devices that enable scientists to increase standardization, precision, and reproducibility of cellular assays. Ananda devices enable up to 50% faster and more precise data analysis in neuroscience, cancer, immunological and stem cells research. We help scientists achieve significant and high quality results faster.
Awards
Founder & CEO
Our work in the news
Pieter Roos has over 15 years experience in the development and commercialization of instrumentation for biomedical research and diagnostics. Prior to joining ANANDA as COO, Pieter worked on bridging the gap in translating new technologies conceived at McGill University into commercial products. As Associate Director of Research & Development at Molecular Biometrics (2007-2011), Pieter was responsible for the design, prototyping and commercial development of their IVF technology platform. Pieter holds a PhD in Analytical Chemistry from Concordia University specialized in microfluidic devices (Montreal, Canada), and completed his Bachelor’s degree in Molecular and Microbial Biology at Larenstein International Agriculture College (Wageningen, the Netherlands).
TEAM
Victoria Mallett is currently pursuing her undergraduate degree at McGill University, expected to graduate next year with a major in Psychology and double minor in Neuroscience and Biology. Victoria has over three years of lab experience as a research assistant at both Stanford University [Dr. Renee Reijo Pera's stem cell lab] and McGill University [Dr. Guy Rouleau's genetics lab]. Victoria also holds the position of Neuroscience Ambassador at McGill's Dobson Centre for Entrepreneurship, where she has helped to build the start-up culture at McGill and in Montreal for the past year and a half. In 2014, Victoria joined ANANDA as the first employee. Victoria is trained in PDMS microfluidic device preparation and completed the McGill X-1 Accelerator with ANANDA in 2015.
Dr Margaret Magdesian, BSc Pharmacology, PhD Biochemistry, has over 15 years of experience in biopharmaceutical research. Tired of the slow, expensive and inefficient process of drug development, in collaboration with researchers from McGill University, she developed a new product to increase standardization, precision, and reproducibility of cellular assays. Recently, she launched a company called ANANDA Devices (www.AnandaDevices.com) to commercialize this product and stimulate other scientists to achieve reproducible and impactful results faster. Dr Magdesian’s innovation has the potential to dramatically accelerate the drug development cycle of new therapies for people that need it.
Pieter Roos, PhD
Margaret Magdesian, PhD
Victoria Mallett
Microdevices to study parasite motility and migration
APPLICATIONS
Microdevices to study cell adhesion and differentiation
Rewiring Neuronal Connections
Microdevices to study neuronal degeneration
Neurons grown on ANANDA's microfluidic chambers can be precisely connected using micromanipulation. Magdesian et al. J.Neuroscience 2016.
Myoblasts (C2C12 line) adhesion and differentiation on netrin-1 microcontact printed stripes (red). Video: S. Ricoult.
Neurons grown on ANANDA's microfluidic chambers connected using atomic force microscopy. Magdesian et al. J.Neuroscience 2016.
Microdevices to study cancer adhesion and invasion
Cancer cells explore the environment sending long membrane protrusions (arrowhead) and are capable of squeezing themselves trying to invade channels much smaller than their own size (arrow). Video: M. Seyed Sadr.
Single hippocampal axons were extended in ANANDA's microfluidic chamber devices, enabling single axonal compression using AFM and subnano forces. Movie shows growth cone retraction, reduction of mitochondria labeling (green), formation of axonal swellings containing mitochondria close and distal from the compression site and final rupture of the axon. Video: Magdesian et al., Biophysical 103(3): 405-14.
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New Technique to Reconnect Neurons
Microfluidic devices
Your Device
ANANDA (Advanced Nano Design Applications) is the result of an interdisciplinary collaboration between cell biologists, physicists and engineers from the Montreal Neurological Institute and the McGill Nanotools Microfab. Our goal is to increase research capacity and competitiveness by developing and applying nano- and microtechnology platforms to improve biomedical research and diagnostics.
Watch our instruction video here
We consult with you to develop custom designs tailored to your research needs. Contact us to learn more about design options and pricing.
We offer Microcontact Printing Stamps in more than 20 different printing patterns to stamp proteins on coverslips and investigate cell adhesion differentiation and growth. Size: Each device is 17mm in diameter and can be used with any coverslip or glass bottom dish > 17mm in diameter.
Microfabrication
We offer Microfluidic Devices that enable cell growth in two different compartments separated by microchannels. These devices can be used in neuroscience to grow axons and soma in different compartments, and in cancer biology, parasitology and immunology to investigate cell migration and chemotaxis. Size: Each device is 17mm in diameter and can be used with any coverslip or glass bottom dish > 18mm in diameter. We usually recommend 25mm coverslips and 35mm plastic dishes with or without glass bottom. The size of the microchannels varies according with the cell type.
We can provide microfabrication services for those who have already developed their own design. Contact us for more information.
Custom Designs
ANANDA’s silicone microdevices enable complete control of the cell microenvironment, providing important insights into cell adhesion, morphogenesis and chemotaxis. ANANDA devices are easy to use and work in any lab conditions, with no need for special coating, coverslips and or microscope adapters. Furthermore, our devices are completely transparent and biocompatible, and allow neurons to survive and be live imaged for up to 28 days. As with recent innovations in miniaturization of electronic devices, miniaturized versions of bioassays offer many advantages, including: * Precise positioning of cells at micrometer distance from each another, contributing to easier and faster microscopy imaging and standardization of cultures and results. * Reduction in the volume of reagents by up to 90% through the use of small cell compartments leading to significant cost reductions for reagents and samples. * Reduction in the number of cells used in each experiment by at least 60%, reducing the use of animals for primary cultures. The smaller volume also helps reduce the time spent on the preparation of cells, recombinant virus and proteins.
In case you have already developed your own designs with us in the past or with anyother company, we can microfabricate more devices.
Micropatterning stamps
Our goal is to improve your lab results by applying micro and nanotechnology to replicate cells’ natural environment in vitro. We offer several types of silicone microFluidic devices for cell culture.
3 X Ananda Devices
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Please note that you can only order 1 sample per customer, any other amount will be ignored.
Each sample package includes :
Yes. Check out the images on our site!
Still need help?
How many cells should I load in each device?
We recommend to use the devices once and use a new device for each application.
Are the devices compatible with organic solvents, such as DMSO or ethanol?
Are the devices cytotoxic?
Yes - please contact us to discuss your interests, custom microfabrication needs or questions!
Can I coat the dishes with PDL, PLL, laminin or any other cell adhesion molecule?
Yes, send us an email with your idea and we can do customized modifications.
Our devices are bio-compatible and cells can grow inside for up to 28 days.
We recommend not to autoclave.
Contact us
It depends on the application and cell type. For DRG neurons we recommend 4,000 cells/device and hippocampal neurons 40,000 cells/device.
Can I fix and stain the cells while they are still in the devices?
Yes, our devices are compatible with DMSO and ethanol.
Yes, you can first coat the dish or coverslip with your favourite substrate, dry and add the devices or assemble the devices first and add the coating solution later. We have multiple protocols for different applications, send us an email and we will be happy to help
Can I re-use the devices?
Can ANANDA provide me with trial samples to check if they work for my cell type?
Could ANANDA change the size of the channels to better accommodate the cell type that I work with?
Please let us know of your question, and we try to answer it as soon as possible.
Can I autoclave the devices?
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Standardizing healthy cell cultures is key to achieve scientific breakthroughs
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● Rapid assembly of functional presynaptic boutons triggered by adhesive contacts. Lucido AL, Suarez Sanchez F, Thostrup P, Kwiatkowski AV, Leal-Ortiz S, Gopalakrishnan G, Liazoghli D, Belkaid W, Lennox RB, Grutter P, Garner CC, Colman DR. J Neurosci. 2009 Oct 7;29(40):12449-66.
Request a free manual from us here
● Xylella fastidiosa outer membrane vesicles modulate plant colonization by blocking attachment to surfaces. Ionescu M, Zaini PA, Baccari C, Tran S, da Silva AM, Lindow SE. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):E3910-8.
Watch the Instructional Video
● Rapid Mechanically-Controlled Rewiring of Neuronal Circuits. Magdesian M, Lopez-Ayon GM, Mori M, Boudreau D, Goulet-Hanssens A, Sanz R, Miyahara Y, Barrett C, Fournier A, De Koninck Y, Grutter P. The Journal of Neuroscience 2016, 36(3), 979-987.
Ananda’s Devices can be used in a wide variety of applications, for a list of example applications.
● Cellular response to micropatterned growth promoting and inhibitory substrates. Belkaid W, Thostrup P, Yam PT, Juzwik CA, Ruthazer ES, Dhaunchak AS, Colman DR. BMC Biotechnol. 2013 Oct 11;13:86.
Learn more
● Characterization of the Xylella fastidiosa PD1671 gene encoding degenerate c-di-GMP GGDEF/EAL domains, and its role in the development of Pierce's disease. Cursino L, Athinuwat D, Patel KR, Galvani CD, Zaini PA, Li Y, De La Fuente L, Hoch HC, Burr TJ, Mowery P. PLoS One. 2015 Mar 26;10(3):e0121851.
● Atomic force microscopy reveals important differences in axonal resistance to injury. Magdesian MH, Sanchez FS, Lopez M, Thostrup P, Durisic N, Belkaid W, Liazoghli D, Grütter P, Colman DR. Biophys J. 2012 Aug 8;103(3):405-14.
Applications
Answers to our most frequently asked questions
● Reversing adhesion with light: a general method for functionalized bead release from cells. Goulet-Hanssens A, Magdesian MH, Lopez-Ayon GM, Grutter P, Barrett CJ. Biomater Sci. 2016 Jul 19;4(8):1193-6.
● Substrate Micropatterning as a New in Vitro Cell Culture System to Study Myelination. Liazoghli D, Roth AD, Thostrup P, Colman DR. ACS Chem Neurosci. 2012 Feb 15;3(2):90-95.
Standardizing healthy cell cultures is key to achieving scientific breakthroughs
With Ananda's silicone microdevices you will be able to:
Request a free Sample
Miniaturization of cellular assays reduces the number of cells needed per experiment, reducing costs of reagents and samples
Increase experimental reproducibility
Perform up to 50% faster and more precise data analysis
Analyze rare samples in a larger number of tests
Every successful experiment relies on high quality cells.
Reproduce cellular in vivo organization in vitro
Neuroscience Immunology Parasitology Cancer Stem cells
Robust cell cultures Reproducible experiments Reliable results
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We help scientists achieve significant and high quality results faster
“Ananda’s easy to use devices offer our lab a new approach to quantify and analyze data at the single-cell level”
Ananda Devices are the first in the market to enable neurite isolation and neuronal survival for longer than 4 weeks in vitro
Maintain high quality cell cultures for several weeks
Ananda Devices adapt to diverse research needs in:
Increase cell culture standardization
You can get more results with fewer cells
Increased reproducibility of neuronal cultures
Easy analysis of axonal Growth
Faster Imaging and Analysis of axonal Degeneration
Ananda Devices facilitate the study and comparison of DRG neuronal growth and development
21 days rat hippocampal neurons labeled for actin red, tubulin green, neurofilament blue
Optimized standardization of cell cultures
Get healthy neuros for several weeks in vitro
Reproducibly organized DRG cultures
Easy to identify and test axons
Easy Identification of Synapses
For the first time neurite isolation and neuronal survival for longer than 4 weeks in vitro. 21 days rat hippocampal neurons labeled for monoglutamylated tubulin in green, polyglutamylated tubulin in magenta, and nuclei in blue. Courtesy of Dr Cecília Rocha de Souza.
Difficult to identify axons
Talk to one of our representatives, and they will take the time to explain to you how this device will work for you
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Bringing research from academia to market
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“… A new way of regenerating the central nervous system”
“Similar techniques may allow to restore function after injury”
Innovation to accelerate drug discovery
How to achieve reproducible and impactful results faster
Margaret Magdesian was a medical researcher who came up with a new way to grow spinal cord cells. The then founded a company, Ananda Devices. (Christinne Muschi For The Globe and Mail)
Scientist & entrepreneur
Innovation to improve people’s life
“New technique offers potential to reconnect neurons of people with central nervous system damage”
read more here
One of the Winners of the 2014 Startup competition in Montreal
2015 Incubator Program
2015 Startup Program
2016 Accelerator Program
2015 Accelerator Program
2015 Startup Program
2016 Finalist of the Global Challenge in Washington DC
Ananda was one of the 7 Finalist selected from 3,500 applicants in 100 countries for the2016 Healthcare Startup Challenge in Paris.
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With Ananda’s device
Ananda Devices are the first in the market to enable neurite isolation and neuronal survival for longer than 4 weeks in vitro
You can get more results with fewer cells
Without Ananda’s device
Every successful experiment relies on high quality cells.
Ambiguous analysis of axonal transport
+
Without Ananda’s device
For the first time neurite isolation and neuronal survival for longer than 4 weeks in vitro. 21 days rat hippocampal neurons labeled for monoglutamylated tubulin in green, polyglutamylated tubulin in magenta, and nuclei in blue. Courtesy of Dr Cecília Rocha de Souza.
Difficult to identify axons
With Ananda’s device
Ambiguous analysis of axonal transport
Our work in the news
Awards
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Tweets by AnandaDevices
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THE FUTURE OF CELL CULTURE IS HERE